| Patent No: | RU2381276 [Russian Federation] | Application No: | RU20060146982 |
| Title: | Agarose and method of its production | ||
| Issue Date: | 10-02-2010 | File Date: | 03-06-2004 |
| Inventor(s): | Siddkhanta Arup Kumar; Meena Ramavatar; Prasad Kamalesh; Ramavat Bkharatkumar Kalidas; Gosh Pushpito Kumar; Ehsvaran Karuppanan; Tiruppati Sangajja; Mantri Vajbkhav Adzhit | ||
| IPC Classification: | C08B 37/00, C12P 19/04 | ||
| Abstract: | FIELD: agriculture. SUBSTANCE: method for production of agarose includes preparation of dry sea algae Gracilaria spp., preferably, Gracilaria dura from natural sources or cultivated in polyethylene bags or on rafts, treatment of dry algae with alkali. Treated algae are washed with water till pH of washing waters makes from 7 to 8, water is added. Autoclaving and further homogenisation are carried out with production of extract. Extract is treated with activated carbon and celite at the temperature of 85-95C to prepare hot extract. Vacuum filtration of extract is carried out through a layer of celite. Filtrate is frozen with preparation of mass, and produced mass is thawed. In order to remove thawed liquid, product produced at the stage of freezing and thawing is compressed and further squeezed. Agarose produced as a result of this process is dissolved in water by means of heating in autoclave. Stage of freezing and thawing, as well as product compressing and squeezing are repeated. Produced product is dissolved in water and dried by means of spraying to produce finely dispersed powder. Agarose is also suggested, which is produced by specified method. ^ EFFECT: invention makes it possible to produce agarose with high strength of gel and low temperature of gel formation Strength of 1% gel makes Ã?Æ?Ã?«Ã?Æ?ââ?¬Ë?1900g/cm2, and gel formation temperature makes 35-35,5C. | ||