Patent Profile
| Patent No: | IN191209 [India] | Application No: | 1192/DEL/99 |
| Title: | A PROCESS FOR THE PREPARATION OF A NOVEL BIOMARKER SPECIFIC FOR O-ACETYLATED SIALIC ACID USEFUL FOR THE DIAGNOSIS, MONITORING OUTCOME OF TREATMENT AND PREDICTION OF RELAPSE AND BIOMARKER PREPARED THEREBY | ||
| Issue Date: | 06-05-2004 | File Date: | 08-09-1999 |
| Inventor(s): | Mandal; Chitra (Indian Institute of Chemical Biology, 4 Raja S | ||
| IPC Classification: | A61K 31/00, G01N 33/50 | ||
| Abstract: | A process for preparation of a novel protein biomarker specific for O-acetylated sialic acid useful for the diagnosis, monitoring outcome of treatment and prediction of relapse which comprises, (i) separating serum from blood collected from patients of acute lymphoblastic leukemia by known methods removing low molecular weight fractions and galactose binding proteins (non-specific protein) from the serum by column chromatography on affinity matrix, (ii) collecting unbound fraction from affinity matrix (iii) passing the galactose free protein fraction obtained in step (ii) over another affinity matrix to capture O-acetyl sialic acid specific protein fraction (iv) eluting specific protein fraction with a buffer at alkaline pH in the range of 8.0-11.0, immediately neutralizing the fraction (v) passing O-acetyl sailic acid specific protein obtained in step (iv) over Protein G-agarose or protein A agarose or protein A Sepharose or protein G Sepharose or anti -human immunoglobulin or only IgG/IgM coupled to Sepharose or agarose column to get O-acetyic sialic acid specific protein immunoglobulin and eluted with a buffer at acidic pH in the range. of 2.0-6.5 immediately neutralizing the fraction and dialyzing to get novel biomarker | ||
CSIR-Indian Institute of Chemical Biology[CSIR-IICB]
: director[at]iicb[dot]res[dot]in
:91-33-24730492
:https://iicb.res.in/
: director[at]iicb[dot]res[dot]in
:91-33-24730492
:https://iicb.res.in/
Industrial Applications
Synhtesis Procesess [Drugs and Pharmaceuticals]
Sustainable Development Goals (SDGs)
Good Health and Well-Being
CSIR-Themes
Agri, Nutrition and Biotech
Healthcare