The present invention relates to a synergistic herbal formulation developed for urolithiasis, nephrolithiasis and post lithotripsy conditions. The formulation includes ingredients from six medicinal plants like Bergenia ciliata, Berberis lycium, Tinospora cordifolia, Phyllanthus neruri, Tribulus terrestris and Coleus forskohlii. The present invention further describes the process of preparation of the synergistic herbal formulation. The formulation is effective in reducing the stones formation in kidney and urinary tract as well as eliminations of stones from kidney, urinary tract and in post lithotripsy conditions and in prevention of the future recurrence.