The  present invention provides a sensitive portable platform device integrated with a thermo-cycler with tunable fluorimeter and DNA probes for meat authentication,  admixing and microbial contaminants. The developed method utilizes genomics-based DNA markers for meat authentication and adulteration in the assessment of the  meat compliance with labeling regulations and policies. The DNA probes are designed to detect target mitochondrial/genomic DNA regions. Individual and multiplex  fluorescence probes for different species have been developed. The developed device is suitable for use by a non-technical person with simplified sample processing  steps and on-the-spot results. The species-specific, processed, and raw meat samples can be differentiated and identified for as low as 0.01% adulteration. The compact  instrumentation with result ready technology/portability will enable regulators to test random samples in the field with high accuracy.