New Invention Profile
| Publication No: | IN202211058469 [India] | Application No: | 202211058469 |
| Title: | A method for linalool synthesis in saccharomyces cerevisiae | ||
| Publication Date: | 19-04-2024 | File Date: | 12-10-2022 |
| Inventor(s): | Sarma Mutturi Venkata Ravi Krishna; Aneesha Abdullah; Nabarupa Gupta; Kalaivani Paramasivan | ||
| IPC Classification: | A61K36/575, C12N15/82, C12R1/865, C12N9/88, C12N15/81 | ||
| Abstract: | The present invention provides a method for linalool synthesis in Saccharomyces cerevisiae,. Here, the linalool synthase gene from Magnolia champaca and Coriandrum sativum were codon optimized, McfLIS: SEQ ID 2 and CsfLIS: SEQ ID 7, respectively, and were expressed in laboratory S. cerevisiae strain CEN.PK2-1C. Moreover, truncated variants of these two linalool synthases were generated (MctLIS and CstLIS) by removal of plastid signal, and expressed in laboratory S. cerevisiae strain CEN.PK2-1C to observe improved linalool synthesis. Also, co-expression of tHMG1 was carried along with McfLIS and MctLIS, on a dual expression plasmid vector using constitutive promoters, pTEF and pPGK1 in S. cerevisiae strain CEN.PK2-1C. | ||
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Industrial Applications
Biotechnology [Biological Science]
Medicinal and Aromatics Plants [Agriculture]
Plants [Biological Science]
Synhtesis Procesess [Drugs and Pharmaceuticals]