New Invention Profile
| Publication No: | IN202411012579 [India] | Application No: | IN202411012579 |
| Title: | A process for the production of Actinomycin D | ||
| Publication Date: | 12-09-2025 | File Date: | 21-02-2024 |
| Inventor(s): | Chaubey, Asha; Sharma, Neha; Manhas, Ravi Singh; Nalli, Yedukondalu; Verma, Mahendra Kumar; Kumar, Amit; Reddy, Dumbala Srinivasa | ||
| IPC Classification: | C07K000706, C07K000120, C12N000120, C12P002102, C12R0001465 | ||
| Abstract: | The present invention describes a fermentation process with specifically designed medium for improved production of pharmaceutically important drug (actinomycin D) using actinobacterial strain Streptomyces marokkonensis MTCC-25511, isolated from cold deserts of NW Himalayas (Barchey) India. The process involves 12 days fermentation under specific fermentation conditions i.e. temperature 28°C±2°C, pH7.0±2.0 with 10% inoculum size followed by downstream processing involving purification of actinomycin D by conventional solvent as well as single step purification method. The direct single step purification involved binding of the metabolites of fermented broth with ion exchange resin and elution of pure compound Actinomycin D using methanol/water/acetone. The present invention provided high productivity i.e. ~15mg/L/h of actinomycin D with 95% purity |
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