The invention describes a series of Benzo[de]isoquinoline-1,3-dione-based fluorophore probes, synthetic processes for the same, and their application for anti-tuberculosis potency and detecting pathogenic or non-pathogenic bacteria from the Mycobacterium genus. The fluorophore probe comprises a dye, a linker, and a benzothiazinone (BTZ)-based targeting group for anchorage to mycobacterial cell wall enzyme DprE1. The dye-BTZ fluorophore probe inhibits the DprE1 enzyme function and further inhibits the growth of Mycobacterium tuberculosis. It allows the signal to be retained and, hence, the single cells of Mycobacterium to be labeled. The conjugate exhibits solvatochromic behavior with respect to the environment. A detectable fluorescent signal is produced by the dye once it is incorporated into the bacterial cell wall because of the change in the local environment.