FnCas9 Editor Linked Uniform Detection Assay (FELUDA)

Detection of pathogenic sequences or variants in DNA and RNA through a point-of-care diagnostic approach is valuable for rapid clinical prognosis. In recent times, CRISPR based detection of nucleic acids have provided an economical and quicker alternative to sequencing-based platforms which are  often difficult to implement in the field. Here, we present FnCas9 Editor Linked Uniform Detection Assay (FELUDA) that employs a highly accurate enzymatic readout for detecting nucleotide sequences, identifying nucleobase identity and inferring zygosity with precision. We demonstrate that FELUDA output can be adapted to multiple signal detection platforms and can be quickly designed and deployed for versatile applications including rapid diagnosis during infectious diseases.

We foresee the commercial application of the knowledgebase would be in the following domains:

1. Clinical diagnosis,
2. Pathogen detections,
3. Panels for group of SNP, mutation detections.
4. Multiple pathogen detections in food
5. Seed quality test
6. DNA test for meat differentiation of cattle and buffalo
7. DNA test for screening of Factor XI Deficiency in cattle and buffalo species
8. DNA test for screening of Citrullinemia in cattle and buffalo species
9. DNA test for screening of Deficiency of uridine monophosphate synthase in cattle and buffalo species
10. DNA test for screening of Bovine Leukocyte Adhesion Deficiency in cattle and buffalo species
11. Assessment of fish quality
12. Assessment of poultry quality 

The knowledgebase encompasses a comprehensive pipeline including protein expression, sgRNA design, preparation of RNA complexes, detection methods that are dependent on applications, SOPs for pathogen detections, SNP detections. This technology has application in fast diagnosis nucleic acid based detection including genomic variations, diseases associated with the variations, pathogens, food contaminants, fish, poultry, cattle qualities