New Invention Profile
| Publication No: | IN202411037383 [India] | Application No: | IN202411037383 |
| Title: | Higher titer biosuccinic acid production by co-substrate fermentation with Citrobacter sp. IICTSVMSA1 and thereof | ||
| Publication Date: | 28-11-2025 | File Date: | 10-05-2024 |
| Inventor(s): | Srinivasulu Reddy Venkata Mohan;Triya Mukherjee;Amulya Kotamraju | ||
| IPC Classification: | C12P7/46, C12N1/20, C12N9/02, C12P7/6409, G09B19/00 | ||
| Abstract: | The present invention discloses a higher titer production of succinic acid using a non-genetic approach with the wild/native strain Citrobacter sp. (NCIM 5782). Succinic acid is a versatile chemical with various industrial applications, and its demand is on the rise as a sustainable alternative to petroleum-based building block. The invention revolves around co-substrate fermentation, specifically the use of a combination of C6 and C3 sugars as carbon sources, which has not been explored extensively before. The optimized conditions involve maintaining a specific ratio of C6 and C3, pH, along with the addition of C1 carbon source, essential for the succinic acid production process. Through careful experimentation, the inventors identified the ideal conditions of the C3:C6 ratio (1: 0.8) that maximize succinic acid production while minimizing the formation of by-products. The process was successfully scaled up to a 25-L fermenter, achieving a remarkable 65-73 g/L of succinic acid (20-26h) with a high productivity rate (3g/L/h; 0.6 g/g), all without any genetic modifications to the strain. This invention not only offers a more sustainable and environmentally friendly method for succinic acid production but also demonstrates the potential of non-genetic approaches as well as co-feedstock addition in enhancing the yield of succinic acid. The disclosed process holds significant promise for the bio-based chemical industry and provides a valuable contribution to the field of industrial microbiology. |
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Industrial Applications
Biotechnology [Biological Science]
Chemistry, Chemical Processes Polymers [Chemistry, Chemical Processes]
Microbial [Biological Science]