In Vitro Protease-Based Sars-Cov-2 Spike Protein Cleavage Assay System

1. It is an in vitro assay system
2. The substrate is purified from bacterial culture and thus purification is easy.
3. Substrate cleavage assay does not require animal or human cell culture
4. Infectious virus is not involved and thus the assay system is safe.
5. The assay system is animal cell culture independent and thus less time consuming
6. The assay system can be upgraded to a high-throughput system and thus it will help in screening multiple drugs at a time.
7. It can also be used to identify if any other unknown host protease involved in spike protein cleavage. The designed assay system is easy to understand and interpret with no complicated steps involved.

The invention relates to development of a reporter based in vitro trypsin/trypsin-like protease-based SARS-CoV-2 spike protein cleavage assay system. The assay system involves a recombinant construct for screening drugs against sars-cov-2 spike protein

The designed assay system is easy to understand and interpret trypsin/trypsin-like protease-based SARS-CoV-2 spike protein cleavage assay systemwith no complicated steps involved.