Indole Based Fluorescent Bio probe For The Super resolution Wash free Live Cell Imaging of Endoplasmic Reticulum

In order to monitor the Endoplasmic Reticulum in Live/ Fixed cells in various cancer/non cancer cells, and deep tissues via confocal imaging the SAG 536 can be widely used. ER stress monitoring can be done with high resolution imaging in live cells.

Novel Indole based probe SAG 536 developed for the rapid and selective detection of intracellular lipophilic Endoplasmic Reticulum (ER) membrane with high contrast
imaging in live cells as well as the first successful example of selective light up probe for distinct ER imaging in live cells.

• Optimal for imaging Live ER Membrane in wash free conditions in vitro
• The developed probe is easy to synthesize, which lowers the cost of available dyes in the market to stain the ER

The existing fluorescence probes for staining intracellular ER suffers with fluorescence crosstalk, high cost, background noise and unable to measure the specific ER membrane in high resolution to understand the ER stress in diseased condition.

Indole based fluorescence probe enables the following features of ER specific probe in live cell:

• High Selectivity 
• Cell membrane permeability in Live and fixed condition
• Low Florescence cross talk
• Highly photostable for high contrast image and 3D imaging of ER membrane.
• Viscous environment sensitive