New Invention Profile
| Publication No: | IN202311015547 [India] | Application No: | IN202311015547 |
| Title: | INTERLEUKIN 1-RECEPTOR ANTAGONIST WITH INCREASED SOLUBILITY AND PROCESS OF PREPARATION THEREOF | ||
| Publication Date: | 13-09-2024 | File Date: | 07-03-2023 |
| Inventor(s): | Rahul Sharad Bhambure; Tejas Prakash Chirmade; Sunil Rana; Neeraj Nainwal | ||
| IPC Classification: | A61K 38/00, C12N 15/70, A61P 37/04, C12P 21/02, C07K 14/54 | ||
| Abstract: | The present invention is directed to the cells, compositions, and methods for the production of and purification of recombinant protein. In particular, the invention is directed to have enhanced soluble expression of interleukin 1-receptor antagonist by means of an optimized novel polynucleotide sequence and host transformed with the said polynucleotide. The invention also provides a method for increased production and purification of interleukin 1-receptor antagonist (IL-1Ra) wherein, the polynucleotide of the present invention is used to transform a suitable host resulting in over-expression of corresponding proteins and a method for isolating the expressed polypeptide. The invention also relates to the purification method for of interleukin 1-receptor antagonist (IL-1Ra) using multimodal chromatography as well as characterization of properly folded of interleukin 1-receptor antagonist (IL-1Ra) protein. |
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CSIR-National Chemical Laboratory[CSIR-NCL]
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Industrial Applications
Biological Science Processes/Methods [Biological Science]
Biomolecules [Biological Science]
Biotechnology [Biological Science]