Novel Process Platform for the Manufacturing and Purification of Anakinra

• Economical production with yield of (1.1 ± 0.20 g/L)
• Simple & cost-effective purification
• Time and cost effective expression avoiding in-vitro refolding of protein
• A two-fold increase in productivity due to integrated multimode chromatography over existing processes

Anakinra is used for the treatment of:

• Rheumatoid arthritis
• Stills disease
• Neonatal-onset multi-system inflammatory disease
• Familial Mediterranean fever

• Novel cloning, expression, refolding & purification platform.
• Upstream and Downstream process developed.
• Soluble expression of Anakinra eliminating in-vitro refolding step.
• Soluble protein expression > 1gm/L of fermentation broth.