New Invention Profile
| Publication No: | IN202411043776 [India] | Application No: | IN202411043776 |
| Title: | Primers and kit for the species specific identification of cinnamomum verum | ||
| Publication Date: | 19-12-2025 | File Date: | 05-06-2024 |
| Inventor(s): | Vandana Jaiswal; Himanshi Gangwar; Vijay Gahlaut; Ramesh Chauhan; Satbeer Singh | ||
| IPC Classification: | C12Q1/6895, A61K36/54, A01N65/24, G06N3/12, C12Q1/6888 | ||
| Abstract: | The present invention aims to develop molecular markers distinguishing Cinnamomum verum (true cinnamon) and Cinnamomum cassia (cassia cinnamon). These species are recognized by morphological, chemical composition and essential oil contents. The appropriate identification of species would be considerably improved by a genetic method. A total 71 ISSR (Inter simple sequence repeat) and four universal barcoding (ITS, rbcL, matK, and psbA-trnH) genes were used to distinguish both the species. No sequence variation was observed between the two species for any DNA barcode gene. However, one ISSR i.e. ISSR-37 showed a clear distinction between the species and produced 570 bp and 746 bp amplicons in C. verum and C. cassia, respectively. The polymorphic bands were converted into species-specific SCAR markers. The SCAR-CV was specific to C. verum and amplified 190 bp band, however there was no amplification seen in the C. cassia samples. The SCAR marker generated in this study can be employed as efficient, economical, and reliable molecular tool for the identification of C. verum. |
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: director[at]ihbt[dot]res[dot]in
:91-1894-230411
:https://www.ihbt.res.in