rRNA Selective Fluorescent Bioprobe For Tracking Viscoelastic Nature of Intracellular Nucleolus

In order to monitor the viscoelastic nature of nucleolus to understand the ribosomal biogenesis during disease progression in Live/ Fixed cells in various cancer/non cancer cells via confocal imaging the NucNK can be widely used.

Hospital, laboratories, the pharmaceutical industry and medical research, vaccine and drug development and disease diagnosis at initial stages, flow cytometry uses dyes to detect or measure the characteristics of cells or tissues.

• Newly designed fluorescence probe of staining live cells for bio imaging, including novel fluorescent cell labelling dye for Lipid droplet imaging and analysis in real time/Live samples.
• Optimal for imaging viscoelastic nature of Live nucleolar rRNA both in vitro and in vivo and bacterial cells. 
• The developed probe is easy to synthesize, which lowers the cost of available dyes in the market to stain nucleolar rRNA.

The existing fluorescence probes for staining nucleolar rRNA suffers with fluorescence crosstalk, high cost, background noise and unable to measure the viscoelastic nature of the nucleolus in diseased condition in real time imaging.

Indole based fluorescence probe enables the following features of viscoelastic nature of nucleolus in live cell. 

• High Selectivity
• Cell membrane permeability in Live and fixed condition
• Low Florescence cross talk
• Viscosity sensitive to monitor minor changes in local
  environment of nucleolus