Technology Profile
| Title: | Technology for Production of Novel High Redox Potential Laccase Enzyme |
| Value Proposition: | A process has been developed for production of a novel high redox potential laccase using a fungal strain isolated in CSIR-IMTECH. |
| Summary Application: | Laccase fermentation is carried out in a special surface culture tray reactor. The laccase produced is extracellular and easy to purify from the broth. Laccase fermentations generally have extended duration making it expensive to run. The CSIR-IMTECH laccase fermentation process is low energy intensive and based on moderate cost not complex medium. The process has been demonstrated on 10 L scale and has yield of 15,000 IU per liter.(1 unit of enzyme activity is the enzyme quantity that produce 1 μM of ABTS cation per min in 50 mM Citrate phosphate buffer, pH 4.0 at 300C and 5 minute incubation time. ). The process can be easily scaled up to semi pilot or pilot scale. |
| Advantages: | Laccase (EC 1.10.3.1) has broad enzyme specificity that imparts high application potential for its applications in various fields such as bioremediation, textile, pulp and paper , food and beverage, cosmetics and organic synthesis. Laccase production is difficult as it is produced in the idiophase when cell metabolism is slow. Low intrinsic production and long fermentation cycle increase laccase production cost. The CSIR-IMTECH process has addressed some issues in the production of laccase and using a novel strain, a reliable process for the production of high redox potential laccase has been developed. The laccase has been tested in industrial environment and has been found to give good performance in denim bleaching. Applications:
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: director[at]imtech[dot]res[dot]in
:91-172-2690550
:https://www.imtech.res.in
